The chromatographic separation of MeOH extract of the aerial parts of Aster glehni (Compositae) led to the isolation of three sesquiterpenes, two sterols and four terpenes. Their structures were determined to be β-amyrin acetate (1), phytol (2), alismol (3), a-tocopheryl quinone (4),7-spinasterol (5), 10-O-methyl alismoxide (6), alismoxide (7), and 3-0-(6`-O-palmitoyl-β-D -glucosyl)- spinasta 7,22-diene (8) by physicochemical and spectroscopic methods . These compounds (1~8) were first isolated from the Aster glehni.