Fig. 3. Increase of autophagic flux by SSRIs in ARPE-19 cells.
ARPE-19 cells were seeded in a 6-well plate at a density of 1×105 cells/well and incubated for 24 h. Cells were treated with SSRIs or co-treated with 10 μM SSRIs and 4 nM bafilomycin A1 for 6 h. Net-LC3-II flux was measured as the increased level of LC3-II levels by bafilomycin A1 treatment in the presence or absence of SSRIs. The results are presented as ±SD (n=3). *p<0.05, ***p<0.001 vs CTR. CTR, control; Cit, Citalopram; Esc, Escitalopram; Fluo, Fluoxetine; Fluv, Fluvoxamine; Par, Paroxetine; Ser, Sertraline.