Fig. 5. Down-regulation of BRD4 target gene expression by BRD4 PROTAC.
(A) RT-qPCR analysis of relative PSA, MYC, TMPRSS2, and KLK2 mRNA levels in LNCaP cells treated with 1 and 10 μM of BRD PROTAC compounds for 24 h. Data are presented as mean ± SD (n=3). (B) LNCaP cells were treated with SD-2406 and SD-2407 (0.01–10 μM) for 48 h. MYC and β-actin protein levels were detected using western blotting. Results are presented as the mean ± S.D. *P<0.05, **P<0.01, ***P<0.001 vs CTR.