Fig. 3. Degradation of BRD4 by PROTAC compounds in LNCaP cells.
(A) LNCaP cells were treated with 0.01–10 μM of BRD4 PROTAC compounds for 24 h. Western blotting was used to measure BRD4 and β-actin protein levels. AT-1 was used as a positive control compound. (B) Changes in relative BRD4 protein levels by PROTAC compound treatment in LNCaP cells. The respective protein levels were quantified using Image J software. (C) BRD4 proteolytic activity of AT-1, SD-2163, SD-2164, SD-2406, and SD-2407 was presented as DC₅₀. (D) BRD4 Degrader AT-1