ISSN 0377-9556 (PRINT)
ISSN 2383-9457 (ONLINE)
ISSN 2383-9457 (ONLINE)
Genes related to carbapenemase and their amplification condition
| Target genes | Primers | Size (bp) | Sequences (5'→3') | Amplification condition |
|---|---|---|---|---|
| blaKPC | KPC F | 893 | ATGTCACTGTATCGCCGTCT | 94°C 5 min+30X (94°C 30 sec+56°C 20 sec+72°C 30 sec)+72°C 7 min |
| KPC R | TTTTCAGAGCCTTACTGCCC | |||
| blaGES | GES F | 753 | GCGCTTCATTCACGCACTAT | |
| GES R | GCGTAATCTCTCTCCTGGGC | |||
| blaNDM-1 | NDM-1 F | 726 | CAATATTATGCACCCGGTCG | |
| NDM-1 R | ATCATGCTGGCCTTGGGGAA | |||
| blaIMP-1 | IMP F | 740 | TGAGCAATGTATCTGTATTC | |
| IMP R | TTAGTTGCTTGGTTTTGATG | |||
| blaVIM | VIM F | 766 | TGGTCTACATGACCGCGTCT | |
| VIM R | CGACTGAGCGATTTGTGTG | |||
| blaOXA-48 | OXA-48 F | 744 | TTGGTGGCATCGATTATCGG | |
| OXA-48 R | GAGCACTTCTTTTGTGATGGC |