약학회지

ISSN 0377-9556 (PRINT)
ISSN 2383-9457 (ONLINE)

Table. 1.

Table. 1. Operating Condition of HPLC
HPLC condition
Instrument Agilent 1260
Column Luna C18 (250×4.6 mm, 5 μm: Phenomenex)
Column Temperature 25°C
Flow rate 1.0 mL/min
Detector Diode array detection WR and multiple wavelength detector
Detector Wavelength 200 nm
Injection volume 10 μL
Mobile phase A: water/phosphoric acid (99.7: 0.3 v/v), B: acetonitrile
Mobile phase condition Time (min) Mobile phase (%)
A B
0 78 22
65 45 55
66 5 95
75 5 95
76 78 22
85 78 22
Yakhak Hoeji 2023;67:398-403 https://doi.org/10.17480/psk.2023.67.6.398
© 2023 Yakhak Hoeji