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Fig. 4.

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Fig. 4. Effect of oleanolic acid on PMA-induced phosphorylation of IKKα/β and IκBα, degradation of IκBα, and phosphorylation/translocation of NF-κB p65 in NCI-H292 cells.
NCI-H292 cells were incubated with varying concentrations of oleanolic acid for 24 h and treated with 50 ng/mL PMA for 30 min. Whole cell lysates (50 μg) were prepared and then subjected to western blot analysis using phosphospecific IKKα/β (Ser 176/180) antibody. Cytoplasmic extracts were fractionated and then subjected to western blot analysis using phosphospecific IκBα (Ser 32/36) antibody or antibody against anti-IκBα. The results shown are the representative of three independent experiments. Equal protein loading was evaluated by β-actin levels (A). Nuclear protein extracts were prepared and subjected to western blot analysis using phospho-specific p65 (Ser 536) antibody and antibody against p65. As a loading control, p84 levels were analyzed. The results shown are the representative of three independent experiments (B). * significantly different from control (p<0.05). + significantly different from PMA alone (p<0.05). cont: control, O: oleanolic acid, O1: oleanolic acid 1 μM, O5: oleanolic acid 5 μM, O10: oleanolic acid 10 μM, O20: oleanolic acid 20 μM, IKKα/β: Inhibitory kappa B kinase α/β, IκBα: inhibitory kappa Bα. concentration unit is μM.
Yakhak Hoeji 2023;67:335-41 https://doi.org/10.17480/psk.2023.67.6.335
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