YAKHAK HOEJI

Table. 1.

Table 1 Gradient conditions of previous method vs modified method

Time (min)	Previous mobile phase			Modified mobile phase
Time (min)	A	B	C	A*	B*
0	81	15	4	85	15
15	66	30	4	70	30
23	16	80	4	20	80
27	16	80	4	20	80
27.1	81	15	4	85	15
35	81	15	4	85	15
Mobile Phase	A: Methanol, B: MTBE, C: 1% phosphoric acid
Mobile Phase	A: 0.05% phosphoric acid in MeOH:Water (9:1), B: MTBE