ISSN 0377-9556 (PRINT)
ISSN 2383-9457 (ONLINE)
ISSN 2383-9457 (ONLINE)
Sequence of M1 macrophage primers for quantitative PCR
| Target | PCR primer sequence 5'→3' |
|---|---|
| IL-1β | Forward - TGGACCTTCCAGGATGAGGACA Reverse - GTTCATCTCGGAGCCTGTAGTG |
| TNF-α | Forward - GGTGCCTATGTCTCAGCCTCTT Reverse - GCCATAGAACTGATGAGAGGGAG |
| GAPDH | Forward - AACTTTGGCATTGTGGAAGG Reverse - ACACATTGGGGGTAGGAACA |
Among the Pro-inflammatory cytokines of M1 macrophages, mRNA expression was confirmed by qPCR using mRNA of TNF-α and IL-1β. GAPDH was used as the house keeping gene.