약학회지
YAKHAK HOEJI
ISSN 0377-9556 (PRINT)
ISSN 2383-9457 (ONLINE)

Fig. 4.

Download original image
Fig. 4. Role of CaCC in the SP-mediated melanogenesis in B16 melanoma cells. In these experiments BAPTA/AM (1 μM), and NFA (100 μM) and FFA (100 μM), were used as an intracellular Ca2+ chelator and inhibitors of CaCCs, respectively. Intracellular Cl concentration was assessed by MQAE fluorescence technique, and the data (A) represent changes in intracellular Cl concentration ([Cl]i) with time. The arrow shows the time point for addition of SP (20 μM). BAPTA/AM, NFA and FFA were given 10 min before SP treatment. In the data (B) and (C), quantitative changes in [Cl]i and melanin contents, respectively, are expressed as a fold increase compared to negative control. *p<0.05 compared to negative control. #p<0.05 compared to SP alone. Abbreviations: BAPTA/AM, bis-(oaminophenoxy)- ethane-N,N,N’,N’-tetraacetic acid/acetoxymethyl ester; CaCC, Ca2+-activated Cl- channels; FFA, flufenamic acid; NFA, niflumic acid; SP, spiperone.
Yakhak Hoeji 2021;65:349-54 https://doi.org/10.17480/psk.2021.65.5.349
© 2021 Yakhak Hoeji